A TETRAZOLIUM SALT REDUCTION METHOD FOR DEMONSTRATING LIPOPROTEINS IN TISSUE SECTIONS

Direct immunoperoxidase method for demonstrating Chlamydia psittaci in tissue sections.

Reduction of a tetrazolium salt in determining growth activity of yeast-phase Histoplasma capsulatum.

A colorimetric method using a tetrazolium compound, 3,4,5-dimethylthiozalil-(1 or 2),2,5-diphenyltetrazolium bromide (TTBr), was developed for studying the growth activity of yeast-phase Histoplasma capsulatum. Materials extracted in phosphate buffer, pH 7.0, from cells at different stages of growth reduced TTBr. Colorimetric changes were correlated with enzymatic activity. Under standardized c...

An allochrome periodic acid-methenamine silver stain for demonstrating mycobacteria in tissue sections.

Nyka9) employed the Gomori-Grocott's chromic acid-methenamine silver method for demonstrating tubercle bacilli in tissue sections after sublimate fixative with added hydrogen peroxide or periodic acid. He described that this procedure could demonstrate mycobacteria that had lost their acidfastness by the usual carbol fuchsin stain. Harada5) also employed the periodic acid-methenamine silver sta...

2D-PCR: a method of mapping DNA in tissue sections.

A novel approach was developed for mapping the location of target DNA in tissue sections. The method combines a high-density, multi-well plate with an innovative single-tube procedure to directly extract, amplify, and detect the DNA in parallel while maintaining the two-dimensional (2D) architecture of the tissue. A 2D map of the gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was created...

Procedure for demonstrating lepra bacilli in paraffin sections.

The lesser degree of acid-fastness of lepra bacilli as compared with tubercle bacilli appears in a disagreeable manner in the difficulty of demonstrating the organisms of leprosy in paraffinized tissues (1). Faraco (2) showed that by ordinary methods of demonstrating acid-fast organisms the lepra bacilli are often not acid-fast, are not differentiated and may be stained by the counterstain. He ...